Abcam’s anti-Chlamydia pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Chlamydia pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Chlamydia pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia pneumoniae IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Chlamydia pneumoniae (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Chlamydia pneumoniae anti-IgM HRP Conjugate||Black cap||1 x 20ml|
|Chlamydia pneumoniae IgM Cut-off Control||Green cap||1 x 3ml|
|Chlamydia pneumoniae IgM Negative Control||Blue cap||1 x 2ml|
|Chlamydia pneumoniae IgM Positive Control||Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgM Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
ab108723 has not yet been referenced specifically in any publications.