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Abcam’s Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum.
A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta antibodies. Samples and standards and are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody. After incubation and washing, added anti-Chorionic Gonadotropin beta HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Chorionic Gonadotropin beta HRP Conjugate||1 x 18ml|
|Chorionic Gonadotropin beta Standard 1 - 0 ng/mL (Lyophilized)||1 vial|
|Chorionic Gonadotropin beta Standard 2 - 2.5 ng/mL (Lyophilized)||1 vial|
|Chorionic Gonadotropin beta Standard 3 - 5 ng/mL (Lyophilized)||1 vial|
|Chorionic Gonadotropin beta Standard 4 - 10 ng/mL (Lyophilized)||1 vial|
|Chorionic Gonadotropin beta Standard 5 - 25 ng/mL (Lyophilized)||1 vial|
|Chorionic Gonadotropin beta Standard 6 - 50 ng/mL (Lyophilized)||1 vial|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
|Zero Buffer||1 x 13ml|
Our Abpromise guarantee covers the use of ab108638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted
Free beta hCG measured in various samples showing concentration in ng/mL
ab108638 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"