Human Chorionic Gonadotropin beta ELISA Kit (ab108638)

Overview

  • Product nameHuman Chorionic Gonadotropin beta ELISA Kit
    See all Chorionic Gonadotropin beta kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.25 ng/ml
  • Range
    0.25 ng/ml - 50 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum.

    A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta antibodies. Samples and standards and are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody. After incubation and washing, added anti-Chorionic Gonadotropin beta HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells) 1 unit
    Anti-Chorionic Gonadotropin beta HRP Conjugate 1 x 18ml
    Chorionic Gonadotropin beta Standard 1 - 0 ng/mL (Lyophilized) 1 vial
    Chorionic Gonadotropin beta Standard 2 - 2.5 ng/mL (Lyophilized) 1 vial
    Chorionic Gonadotropin beta Standard 3 - 5 ng/mL (Lyophilized) 1 vial
    Chorionic Gonadotropin beta Standard 4 - 10 ng/mL (Lyophilized) 1 vial
    Chorionic Gonadotropin beta Standard 5 - 25 ng/mL (Lyophilized) 1 vial
    Chorionic Gonadotropin beta Standard 6 - 50 ng/mL (Lyophilized) 1 vial
    Stop Solution 1 x 11ml
    TMB Substrate Solution 1 x 11ml
    Zero Buffer 1 x 13ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Chorionic Gonadotropin beta ELISA Kit images

  • Colorimetric standard curve: mean of duplicates (+/-) with background readings subtracted

  • Free beta hCG measured in various samples showing concentration in ng/mL

Protocols

References for Human Chorionic Gonadotropin beta ELISA Kit (ab108638)

ab108638 has not yet been referenced specifically in any publications.

Product Wall

Abreviews

Sample: Human Recombinant protein (Culture media collected from human colorectal cell)
Specification: Culture media collected from human colorectal cell
Amount of Sample: .002-.050 µg
Type of Kit Used: ELISA
Positive control: Spiked fresh culture media and mouse urine with protein provided for standard curves
Did you use the Abcam Kit protocol?: Yes
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Abcam user community

Verified customer

Submitted Mar 11 2013

Unfortunately we don't have any testing information for urine and cell culture media. It may work for these samples, but unfortunately we just don't know for sure.

This kit has been developed and validated for use on human plasma and serum samples. We have not tested the use of this kit on mouse urine or cell culture media, and unfortunately we don't know if it would work.

Abreviews

Sample: Human Cell (Epithelial ovarian cancer)
Specification: Epithelial ovarian cancer
Amount of Sample: 20 µg
Type of Kit Used: ELISA
Positive control: Conditioned media from BEWO cells which are known to secrete Chorionic Gonadotropin beta.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Chorionic Gonadotropin beta could be detected from conditioned media without having to concentrate the media. However, if collecting media from a few cells, a longer incubation time or concentrating the media may be necessary.
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Snega Sinnappan

Verified customer

Submitted May 02 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"