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Abcam’s Chorionic Gonadotropin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin in Human serum.
A 96-well plate has been precoated with anti-Chorionic Gonadotropin antibodies. Samples and standards and are added to the wells, where Chorionic Gonadotropin in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Chorionic Gonadotropin HRP conjugate binds to the antibody-Chorionic Gonadotropin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Chorionic Gonadotropin Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Chorionic Gonadotropin HRP Conjugate||1 x 18ml|
|Chorionic Gonadotropin Standard 0 - 0 mU/mL||1 vial|
|Chorionic Gonadotropin Standard 1 - 5 mU/mL||1 vial|
|Chorionic Gonadotropin Standard 2 - 20 mU/mL||1 vial|
|Chorionic Gonadotropin Standard 3 - 50 mU/mL||1 vial|
|Chorionic Gonadotropin Standard 4 - 150 mU/mL||1 vial|
|Chorionic Gonadotropin Standard 5 - 300 mU/mL||1 vial|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
|Zero Buffer||1 x 13ml|
Our Abpromise guarantee covers the use of ab108636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108636 has not yet been referenced specifically in any publications.