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Abcam's Clostridium tetani toxin 5S IgG Human ELISA Kit is intended for the quantitative determination of IgG class antibodies against Clostridium tetani toxin in human serum or plasma (citrate).
Microtiter strip wells are precoated with inactivated Tetanus toxoid antigens to bind coresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured Tetanus toxoid specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
Clostridia are spore-forming gram-positive bacteria. The round spores develop at the terminal end which results in a “tennis racket” like shape under the microscope. Tetanus develops only when spores of Clostridium tetani germinate under strict anaerobic conditions after gaining access to wounds and small lacerations. The clinical manifestation of the disease is not caused by the invasion of the exciter but by the secretion of a powerful neurotoxin (tetanospasmin). This toxin blocks the inhibition of the signal transduction and has a high affinity to the central nervous system. The consequence is hyper excitability of the muscles to external stimuli in combination with a principal increase of the muscle tonus without influence of consciousness.
Clostridium tetani is ubiquitous present in soil and intestine of humans and animals. Ingestion of bacteria or growth in the intestine of man or animal is without harm. The spores are extremely resistant towards heat and can stay infectious for a long period. The bacteria can get under the skin by even the smallest of wounds.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Clostridium tetani toxin 5S IgG Coated Microplate (12 x 8 wells)||1 unit|
|Clostridium tetani toxin anti-IgG Conjugate||Blue, Black cap||1 x 20ml|
|Clostridium tetani toxin IgG Standard A - 0.0 U/mL||Blue cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard B - 0.1 U/mL||Green Cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard C - 0.5 U/mL||Green Cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard D - 1.0 U/mL||Red Cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard E - 5.0 U/mL||White Cap||1 x 2ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108772 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
Representative Standard Curve using ab108772.
ab108772 has not yet been referenced specifically in any publications.