|Weak + Serum||24||0.56U/ml||7.1%|
|Weak + Serum||10||0.22U/ml||11.3%|
Abcam’s anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.
A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Clostridium tetani toxin (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Clostridium tetani toxin anti-IgG HRP Conjugate||Blue, Black cap||1 x 20ml|
|Clostridium tetani toxin IgG Standard A – 0.0 U/mL||Blue cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard B – 0.1 U/mL||Green Cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard C – 0.5 U/mL||Yellow cap||1 x 2ml|
|Clostridium tetani toxin IgG Standard D – 1.0 U/mL||Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
Representative Standard Curve using ab108771
ab108771 has not yet been referenced specifically in any publications.