Human colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 59 years) (ab44191)


  • Product nameHuman colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 59 years)
    See all Colon tissue lysate lysates
  • Description
    Human colon insoluble fraction lysate
  • General notesTissue: Colon Location: Sigmoid colon. Diagnosis: Adenocarcinoma, moderately differentiated. Stage: III T3N1M0 Grade: II Sex: Female Age: 59 years Gross findings: Tumor size 5 x 4 x 2 cm with ulcerative surface. Cut section is soft, white. Regional lymph nodes: 9. Microscopic findings: Tissue sections show proliferation of malignant epithelial cell clusters. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Mitotic figures are evident. The tumor cells form glands and invaded the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show positive (4/9). Insoluble protein fraction

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44191: Extraction 1: soluble protein fraction ab44189 Human colon tumour tisue lysate ab44188 Human colon normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab44191 Human colon tumour tissue lysate ab44190 Human colon normal tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 59 years) (ab44191)

    ab44191 has not yet been referenced specifically in any publications.

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