Human colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 74 years) (ab44199)

Overview

  • Product nameHuman colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 74 years)
    See all Colon tissue lysate lysates
  • Description
    Human colon insoluble fraction lysate
  • General notesTissue: Colon Location: Not recorded. Diagnosis: Adenocarcinoma, well differentiated. Stage: IIIA T2N1M0 Grade: II Sex: Female Age: 74 years Appearance: Size: 3 x 3 x 4 cm. Color: Red-dark. Consistency: Soft Cut surface Non-identical. Hemorrhagic. Presence of Lymphocytic infiltration, Necrosis, Fibrosis, Cystic degeneration. Cellular differentiation: Adenomatous Glandular cells and Glandular formation. Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44199: Extraction 1: soluble protein fraction ab44196 Human colon tumor tissue lysate ab44195 Human colon normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab44199 Human colon tumor tissue lysate ab44197 Human colon normal tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature

    References for Human colon tissue lysate (adenocarcinoma) - insoluble fraction (female, 74 years) (ab44199)

    ab44199 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"