Human colon tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years) (ab44174)


  • Product nameHuman colon tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years)
    See all Colon tissue lysate lysates
  • Description
    Human colon soluble fraction lysate
  • General notesTissue: Colon Location: Rectum Diagnosis: Adenocarcinoma, well differentiated Stage: II T3NoMo Grade: I Sex: Female Age: 46 years Gross findings: Tumor size 4 x 3 x 2 cm. Cut section is soft, white. Regional lymph nodes: 3. Microscopic findings: Tissue sections show proliferation of malignant epithelial glands. Epithelial cells have small basophilic, irregular nuclei and abundant cytoplasm. Cytoplasm contains muscin. Tumor cells arranged in glands. Mitotic figures are not evident. The tumor cells invaded the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show hyperplasia. Soluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44174: Extraction 1: soluble protein fraction ab44174 Human Colon Tumor Tissue lysate ab44173 Human Colon Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44176 Human Colon Tumor Tissue lysate ab44175 Human Colon Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human colon tissue lysate (adenocarcinoma) - soluble fraction (female, 46 years) (ab44174)

    ab44174 has not yet been referenced specifically in any publications.

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