General notesTissue: Colon
Location: Sigmoid colon.
Diagnosis: Adenocarcinoma, moderately differentiated.
Stage: III T3N1M0
Age: 59 years
Gross findings: Tumor size 5 x 4 x 2 cm with ulcerative surface. Cut section is soft, white. Regional lymph nodes: 9.
Microscopic findings: Tissue sections show proliferation of malignant epithelial cell clusters. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Mitotic figures are evident. The tumor cells form glands and invaded the muscularis propria and subserosa.
The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are not revealed. Lymph nodes show positive (4/9).
Soluble protein fraction
This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44189:
Extraction 1: soluble protein fraction
ab44189 Human colon tumour tisue lysate
ab44188 Human colon normal tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44191 Human colon tumour tissue lysate
ab44190 Human colon normal tissue lysate (matched)
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature