Human colon tissue lysate (adenocarcinoma) - soluble fraction (male, 49 years) (ab42066)


  • Product nameHuman colon tissue lysate (adenocarcinoma) - soluble fraction (male, 49 years)
    See all Colon tissue lysate lysates
  • Description
    Human colon soluble fraction lysate
  • General notes

    Tissue: Colon Location: Not recorded. Diagnosis: Adenocarcinoma, moderately differentiated. Stage: II N0M0 Grade: II Gross findings: Tumor size 3 x 3 x 2 cm with ulcerative surface. Cut surface is soft, white. Paracolic lymph nodes: 2. Microscopic findings: Tissue sections show proliferation of malignant epithelial cell clusters. Epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Mitotic figures are evident. The tumor cells form glands and invaded through the muscularis propria and subserosa. The stroma is infiltrated by large numbers of inflammatory cells. Tumor necrosis and blood vessel invasion are revealed. Lymph nodes show negative.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42066: Extraction 1: soluble protein fraction ab42066 Human colon tumor tissue lysate ab42065 Human colon normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab42069 Human colon tumor tissue lysate ab42067 Human colon normal tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human colon tissue lysate (adenocarcinoma) - soluble fraction (male, 49 years) (ab42066)

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