Human colon tissue lysate - insoluble fraction (male, 25 years) (ab44142)

Overview

  • Product nameHuman colon tissue lysate - insoluble fraction (male, 25 years)
    See all Colon tissue lysate lysates
  • General notes

    Diagnosis: Normal.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insouble protein fraction. Please find below a range of products that relate to ab44142: Extraction 1: soluble protein fraction ab44141 Human colon tumor tissue lysate ab44140 Human colon normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab44143 Human colon tumor tissue lysate ab44142 Human colon normal tissue lysate (matched).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human colon tissue lysate - insoluble fraction (male, 25 years) (ab44142)

    ab44142 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"