Overview

  • Product name
    Human Complement C1q ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Plasma 1 20 4.8%
    Plasma 2 20 4.5%
    Plasma 3 20 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 1 20 10.4%
    Plasma 2 20 10.1%
    Plasma 3 20 9.7%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.03 ng/ml
  • Range
    0.039 ng/ml - 40 ng/ml
  • Recovery

    = 97.5 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Cow, Pig
  • Product overview

    Complement C1q Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab170246) is designed for the quantitative measurement of Complement C1q concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.


    A Complement C1q specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1q specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1q captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C1q Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C1q Microplate (12 x 8 well strips) 1 unit
    Complement C1q Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease
    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities
    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications
    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • C1qa
    • C1QA_HUMAN
    • Complement C1q subcomponent subunit A
  • Database links

Applications

Our Abpromise guarantee covers the use of ab170246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab170246

Protocols

References

This product has been referenced in:
  • Ting KR  et al. Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients. BBA Clin 8:28-34 (2017). Read more (PubMed: 28725572) »
  • McGuire JL  et al. The complement system, neuronal injury, and cognitive function in horizontally-acquired HIV-infected youth. J Neurovirol 22:823-830 (2016). ELISA ; Human . Read more (PubMed: 27273074) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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