Overview

  • Product name
    Human Complement C1r ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.8%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.1 ng/ml
  • Range
    0.125 ng/ml - 8 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement C1r Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C1r concentrations in plasma, serum, saliva, urine, milk and cell culture supernatants.

    A Complement C1r specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1r specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1r captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C1r Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C1r Microplate (12 x 8 well strips) 1 unit
    Complement C1r Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Post-translational
    modifications
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt
  • Alternative names
    • C1R
    • C1R_HUMAN
    • Complement C1r subcomponent
    • Complement C1r subcomponent light chain
    • Complement component 1 r subcomponent
    • Complement component 1 subcomponent r
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab170245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab170245

Protocols

References

ab170245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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