Overview

  • Product nameHuman Complement C3 ELISA Kit
    See all Complement C3 kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.6 ng/ml
  • Range
    0.625 ng/ml - 40 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Complement C3 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.

    A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionC3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    Acylation stimulating protein (ASP): adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.
  • Tissue specificityPlasma. The acylation stimulating protein (ASP) is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods.
  • Involvement in diseaseComplement component 3 deficiency
    Age-related macular degeneration 9
    Hemolytic uremic syndrome atypical 5
    Increased levels of C3 and its cleavage product ASP, are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.
  • Sequence similaritiesContains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by insulin and dietary chylomicrons.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • ASP
    • C3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • C3a anaphylatoxin
    • C3adesArg
    • CO3_HUMAN
    • Complement C3
    • Complement C3 alpha chain
    • Complement C3b alpha' chain
    • Complement C3c alpha' chain fragment 1
    • Complement C3c alpha'' chain fragment 2
    • Complement C3d fragment
    • Complement C3dg fragment
    • Complement C3f fragment
    • Complement C3g fragment
    • CPAMD1
    • omplement C3 beta chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Complement C3 ELISA Kit images

  • Complement C3 measured in various samples showing quantity (microgram) per mL of tested sample

  • Representative Standard Curve using ab108823

Protocols

References for Human Complement C3 ELISA Kit (ab108823)

ab108823 has not yet been referenced specifically in any publications.

Product Wall

The first assay you asked about, ab108823, is that kind of assay (sandwich), but the secondary (detection) antibody is biotinylated, not conjugated to an enzyme. The enzyme (HRP) is conjugated to streptavidin, which binds to the biotin on the antibody....

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A better choice for plasma samples is ab108822.


Complement C3 is very abundant in plasma/serum samples. We have developed two different assays.

ab108823 is a sensitive sandwich ELISA assay. If you use this kit for plasma or serum sample, it requires very large dilution. Normal plasma C...

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Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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I am sorry to hear that you have experienced so many problems with the Complement C3 Human ELISA kit (ab108823). I appreciate the time you took to provide further details.

After reviewing the provided information I suggest to verify the sample...

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Thank you for contacting us.

I have been in touch with the lab to inquire aboout use ofab108823 Complement C3 Human ELISA kit with plasma.This kit can be used for plasma, but you need to find out the appropriate dilution factor. We suggest...

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Muchas gracias por tu respuesta.

Actualmente tenemos una promoción para España por la que se aplica el 25% de descuento a todos los productos que no son anticuerpos primarios. La información sobre la promoción est&...

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Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apro...

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Gracias por contactar con abcam.

Me gustaría informarle que tenemos 2 kits para la deteccion de humano C3 (tambien llamado ASP).

Las referencias sonab108822 (para Serum, Plasma) and ab108823 (para Cell culture supernatant, Sa...

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Thank you for contacting us.

Unfortunately, the lab does not have any information regarding the isoelectric point for this kit. I am sorry I could not be more helpful, please let me know if you need any additional information.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"