Human Anti-Corynebacterium diphtheriae toxin 5S plus IgG ELISA Kit (ab108761)

Overview

  • Product nameHuman Anti-Corynebacterium diphtheriae toxin 5S plus IgG ELISA Kit
    See all Corynebacterium diphtheriae toxin 5S IgG kits
  • Detection methodColorimetric
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Sensitivity
    0.01 U/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Corynebacterium diphtheriae toxin 5S plus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin 5S in Human serum and plasma.

    A 96-well plate has been precoated with Corynebacterium diphtheriae toxin 5S antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin 5S-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin 5S IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Corynebacterium diphtheriae toxin 5S (IgG) Coated Microplate (12 x 8 wells) 12 x 8 tests
    Corynebacterium diphtheriae toxin 5S anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml
    Corynebacterium diphtheriae toxin 5S IgG Additional Control Yellow, Orange cap 1 x 2ml
    Corynebacterium diphtheriae toxin 5S IgG Standard A - 0.000 U/mL Yellow, Blue cap 1 x 2ml
    Corynebacterium diphtheriae toxin 5S IgG Standard B - 0.015 U/mL Yellow, Green Cap 1 x 2ml
    Corynebacterium diphtheriae toxin 5S IgG Standard C - 0.075 U/mL Yellow, Yellow cap 1 x 2ml
    Corynebacterium diphtheriae toxin 5S IgG Standard D - 0.150 U/mL Yellow, Red cap 1 x 2ml
    Corynebacterium diphtheriae toxin 5S IgG Standard E - 1.000 U/mL Yellow, White cap 1 x 2ml
    Cover foil 1 unit
    IgG Sample Diluent Yellow, White cap 1 x 100ml
    Stop Solution Red cap 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab108761 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Human Anti-Corynebacterium diphtheriae toxin 5S plus IgG ELISA Kit images

    • Representative Standard Curve using ab108761

    Protocols

    References for Human Anti-Corynebacterium diphtheriae toxin 5S plus IgG ELISA Kit (ab108761)

    ab108761 has not yet been referenced specifically in any publications.

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    Thank you for contacting us for your patience in this matter.

    The Diphtheria Control inab108761 lot#GR81621-1is at 0.27 IU/ml with a range of 0.15-0.40 IU/ml.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"