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Abcam’s anti-Corynebacterium diphtheriae toxin 5S plus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin 5S in Human serum and plasma.
A 96-well plate has been precoated with Corynebacterium diphtheriae toxin 5S antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin 5S-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin 5S IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Corynebacterium diphtheriae toxin 5S (IgG) Coated Microplate (12 x 8 wells)||12 x 8 tests|
|Corynebacterium diphtheriae toxin 5S anti-IgG HRP Conjugate||Blue, Black cap||1 x 20ml|
|Corynebacterium diphtheriae toxin 5S IgG Additional Control||Yellow, Orange cap||1 x 2ml|
|Corynebacterium diphtheriae toxin 5S IgG Standard A - 0.000 U/mL||Yellow, Blue cap||1 x 2ml|
|Corynebacterium diphtheriae toxin 5S IgG Standard B - 0.015 U/mL||Yellow, Green Cap||1 x 2ml|
|Corynebacterium diphtheriae toxin 5S IgG Standard C - 0.075 U/mL||Yellow, Yellow cap||1 x 2ml|
|Corynebacterium diphtheriae toxin 5S IgG Standard D - 0.150 U/mL||Yellow, Red cap||1 x 2ml|
|Corynebacterium diphtheriae toxin 5S IgG Standard E - 1.000 U/mL||Yellow, White cap||1 x 2ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Yellow, White cap||1 x 100ml|
|Stop Solution||Red cap||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
Representative Standard Curve using ab108761
ab108761 has not yet been referenced specifically in any publications.