Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit (ab108726)

Overview

  • Product nameHuman Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Weak Pos. 24 12.1%
    Positive 24 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Weak Pos 12 0.04U/ml 11.4%
    Positive 12 0.16U/ml 2.8%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Sensitivity
    = 0.01 U/ml
  • Range
    0.01 IU/ml - 0.15 IU/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Corynebacterium diphtheriae toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin in Human serum or plasma.

     

    A 96-well plate has been precoated with Corynebacterium diphtheriae toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108726 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit images

    • Representative Standard Curve Using ab108726

    Protocols

    References for Human Anti-Corynebacterium diphtheriae toxin IgG ELISA Kit (ab108726)

    ab108726 has not yet been referenced specifically in any publications.

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