Abcam’s anti-Corynebacterium diphtheriae toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Corynebacterium diphtheriae toxin in Human serum or plasma.
A 96-well plate has been precoated with Corynebacterium diphtheriae toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Corynebacterium diphtheriae toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Corynebacterium diphtheriae toxin IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|C. diphtheriae toxin IgG Standard A - 0 U/mL||1 x 2ml|
|C. diphtheriae toxin IgG Standard B - 0.015 U/mL||1 x 2ml|
|C. diphtheriae toxin IgG Standard C - 0.075 U/mL||1 x 2ml|
|C. diphtheriae toxin IgG Standard D - 0.150 U/mL||1 x 2ml|
|Corynebacterium diphtheriae toxin (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Corynebacterium diphtheriae toxin anti-IgG HRP Conjugate||Colored blue, black cap||1 x 20ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Colored yellow, white cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
Representative Standard Curve Using ab108726
ab108726 has not yet been referenced specifically in any publications.