Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceAn 18 amino acid synthetic peptide whose sequences are derived from human CPEB protein. The sequence of this peptide is (amino to carboxy terminus): CH(545)SMEGLRHHSPLMRNQKN(562).
    • Amino acids545 to 562

Associated products

Specifications

Our Abpromise guarantee covers the use of ab4988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • FormLiquid
  • Additional notes


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and the human CPEB, ab3465. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names
    • CEBP
    • CPE binding protein 1
    • CPE BP1
    • CPE-binding protein 1
    • CPE-BP1
    • CPEB
    • CPEB 1
    • CPEB-1
    • CPEB1
    • CPEB1 protein
    • CPEB1_HUMAN
    • Cytoplasmic polyadenylation binding protein 1
    • Cytoplasmic polyadenylation element binding protein
    • Cytoplasmic polyadenylation element binding protein 1
    • Cytoplasmic polyadenylation element-binding protein 1
    • FLJ13203
    • h CEBP
    • h-CEBP
    • hCPEB 1
    • hCPEB-1
    • MGC34136
    • MGC60106
    • mKIAA0940
    • MKIAA0940 protein
    see all
  • FunctionSequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress.
  • Tissue specificityIsoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.
  • Sequence similaritiesBelongs to the RRM CPEB family.
    Contains 2 RRM (RNA recognition motif) domains.
  • DomainThe 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA.
  • Post-translational
    modifications
    Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA.
  • Cellular localizationCytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs.
  • Information by UniProt

References for Human CPEB1 peptide (ab4988)

ab4988 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. The CPEB (cytoplasmic polyadenylation element binding protein) peptide was created to be used as an immunogen for our anti-CPEB antibody. It can be used to neutralize the protein detecting CPEB in control experiments. We do ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"