• Product name
    Human Cu/Zn Superoxide Dismutase ELISA Kit
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 5.1%
    Sample n Mean SD CV%
    Overall 5.8%
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.04 ng/ml
  • Range
    0.08 ng/ml - 5 ng/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Serum 98 89% - 108%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cu/Zn Superoxide Dismutase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in Cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.

    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • Notes

    ab119520 Cu/Zn Superoxide Dismutase Human ELISA kit has been shown to detect both SOD1 and SOD3.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Function
    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
  • Involvement in disease
    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.
  • Sequence similarities
    Belongs to the Cu-Zn superoxide dismutase family.
  • Post-translational
    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.
  • Cellular localization
    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt
  • Alternative names
    • ALS
    • ALS1
    • Cu/Zn superoxide dismutase
    • homodimer
    • hSod1
    • Indophenoloxidase A
    • IPOA
    • SOD
    • SOD soluble
    • SOD1
    • SOD3
    • Superoxide dismutase [Cu-Zn]
    • Superoxide dismutase 1
    • Superoxide dismutase 1 soluble
    • Superoxide dismutase 3
    • Superoxide dismutase cystolic
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Cu/Zn Superoxide Dismutase ELISA Kit images

  • Representative Standard Curve using ab119520.


References for Human Cu/Zn Superoxide Dismutase ELISA Kit (ab119520)

This product has been referenced in:
  • Stradling JR  et al. Biomarkers of oxidative stress following continuous positive airway pressure withdrawal: data from two randomised trials. Eur Respir J 46:1065-71 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26022961) »

See 1 Publication for this product

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Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with sampl...

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Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: http://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: http...

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