• Product name
    Human CXCL5 ELISA Kit
    See all CXCL5 kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    > 92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 102.15 86% - 106%
    Serum 93.39 82% - 102%
    Plasma 92.15 81% - 101%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CXCL5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL5 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CXCL5 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CXCL5 (lyophilized) 2 vials
    CXCL5 Microplate (12 x 8 wells) 1 unit
    Recombinant Human CXCL5 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    CXCL5 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. CXCL5 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
  • Alternative names
    • AMCF II
    • AMCFII
    • C X C motif chemokine 5
    • Chemokine (C X C motif) ligand 5
    • CXCL 5
    • ENA 78
    • ENA78
    • Epithelial derived neutrophil activating protein 78
    • GCP 2
    • LIX
    • Neutrophil activating peptide ENA 78
    • Neutrophil activating protein 78
    • SCYB5
    • Scyb6
    • Small inducible cytokine B5
    • Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78)
    • Small inducible cytokine subfamily B, member 15
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • TYPICAL STANDARD CURVE - Assay DIluent A – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
  • TYPICAL STANDARD CURVE - Assay Diluent B – Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.



This product has been referenced in:
  • Asemota OO  et al. Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians. N Am J Med Sci 6:84-8 (2014). ELISA ; Human . Read more (PubMed: 24695523) »
  • Zhou SL  et al. CXCL5 contributes to tumor metastasis and recurrence of intrahepatic cholangiocarcinoma by recruiting infiltrative intratumoral neutrophils. Carcinogenesis 35:597-605 (2014). Read more (PubMed: 24293410) »

See all 2 Publications for this product

Customer reviews and Q&As

CXCR5 concentration in 16 human serum samples

Excellent Excellent 5/5 (Ease of Use)
Human sera were diluted 10 times by Diluent A.

Abcam user community

Verified customer

Submitted Jun 04 2013

Merci de nous avoir contactés.

En attendant votre nouveau kit, je tiens à vous envoyer quelques autres conseils pour l’utilisation de ce kit :

Anticorps de détection - Veuillez centrifuger avant la reconst...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu.

Nous en avons qu’un autre lot en stock donc, je propose dans un pre...

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Merci pour vos réponses. Apres avoir examiné ce cas, j’estime que les Manips 1, 3 et 4 ont donné de bons résultats. Les seules variabilités observées entre ces standards sont dues à l’utilisa...

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Merci d’avoir patientez, je suis toujours en contacte avec le laboratoire. J’essaye d’organiser l’envoi de quelques remplacements gratuits pour ces kits d’un lot qui fonctionne bien. Je suis désolée de vous d...

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Merci pour ces suppléments d’informations. J’ai envoyé vos réponses au laboratoire qui m’ont répondu le suivant :

Je crois que la dégradation de ˜ 50% du signal pour la deuxième mani...

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Merci de nous avoir contactés.

Je suis en contacte avec le laboratoire.

Pour nous aider dans notre enquête, pouvez-vous répondre aux questions suivantes:

1. Quelles étaient les dates pour chaque...

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Thank you for your enquiry.

I can confirm we are able to provide a discount if you order 5 items or more on 1 order.

I would appreciate if you could confirm how many kits you would like to order, and if you have any other items to...

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Veuillez trouver ci-après la réponse que j'ai obtenu du labo concernant le problème rencontré avec les kits ELISA ab100580 et ab100506. Laréponse est en anglais, j'espère que cela ne vousdérange pas. Tha...

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Merci de m'avoir envoyé le fichier Excel avec vos résultats. J'ai noté que seul l'axe absices (concentrations pg/ml) était logarythmique, mais pas l'axe des DO à 450nm. pourriez-vous s'il vous plait refaire et m'envo...

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