Human CXCL7 ELISA Kit (ab100613)
Key features and details
- Sensitivity: 8.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human CXCL7 ELISA Kit
See all CXCL7/PBP kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 8.5 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
93 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.28 84% - 103% Serum 92.76 82% - 102% Plasma 93.45 83% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CXCL7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL7 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL7 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CXCL7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Notes
Optimization may be required with urine samples.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CXCL7 2 vials CXCL7 Microplate (12 x 8 wells) 1 unit Recombinant Human CXCL7 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- B TG1
- Beta TG
- Beta thromboglobulin
see all -
Database links
- Entrez Gene: 5473 Human
- Omim: 121010 Human
- SwissProt: P02775 Human
- Unigene: 2164 Human
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab100613 has been referenced in 4 publications.
- Kollikowski AM et al. Platelet Activation and Chemokine Release Are Related to Local Neutrophil-Dominant Inflammation During Hyperacute Human Stroke. Transl Stroke Res 13:364-369 (2022). PubMed: 34455571
- Li C et al. Potential Markers from Serum-Purified Exosomes for Detecting Oral Squamous Cell Carcinoma Metastasis. Cancer Epidemiol Biomarkers Prev 28:1668-1681 (2019). PubMed: 31350263
- Du Q et al. CTAPIII/CXCL7: a novel biomarker for early diagnosis of lung cancer. Cancer Med 7:325-335 (2018). PubMed: 29356357
- Alsmadi NZ et al. Constricted microfluidic devices to study the effects of transient high shear exposure on platelets. Biomicrofluidics 11:064105 (2017). PubMed: 29204246