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The Human Cyclophilin F (PPIF) ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab221202) is designed for detection of human PPIF in plasma, serum, and cell culture samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PPIF in 4 hours. A polyclonal antibody specific for human PPIF has been pre-coated onto a 96-well microplate with removable strips. PPIF in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for PPIF, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Cyclophilin F, also known as peptidyl-prolyl cis-trans isomerase F (PPIF, PPIase F), cyclophilin D (CyP-D, CypD), mitochondrial cyclophilin (CyP-M), or rotamase F, belongs to the cyclophilin-type PPIase family. As a mitochondrial matrix protein, it is encoded by PPIF gene and the mature protein consists of 178 amino acids. Cyclophilin F catalyzes the cis-trans isomerization of certain proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. It forms part of the mitochondrial permeability transition pore in the inner mitochondrial membrane and plays an essential role in opening the pore leading to apoptosis or necrosis
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 20ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|Anti-Human PPIF coated Microplate (12 x 8 wells)||1 x 96 tests|
|Biotinylated Human PPIF||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Human PPIF Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab221202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab221202 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"