Overview

  • Product nameHuman Cytochrome c ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 4%
  • Tests
    1 x 96 test
  • Sample type
    Cell Lysate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.05 ng/ml
  • Range
    0.08 ng/ml - 5 ng/ml
  • Recovery

    82 %

    Sample specific recovery
    Sample type Average % Range
    Cell Lysate 98 78% - 119%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cytochrome c Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cytochrome c concentrations in cell lysate.

    Cytochrome c specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Cytochrome c detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytochrome c captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • Cellular localizationMitochondrion matrix
  • Alternative names
    • CYC
    • CYCS
    • Cytochrome c somatic
    • HCS
    • THC4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Cytochrome c ELISA Kit images

  • Representative Standard Curve using ab119521.

Protocols

References for Human Cytochrome c ELISA Kit (ab119521)

ab119521 has not yet been referenced specifically in any publications.

Product Wall



The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the...

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Unfortunately, we would not recommend using the Cytochrome c Human ELISA Kit (with color giving dyes), ab119521 with serum sample as when we have tried this we have observed false pos...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"