Human Cytokeratin 14 full length protein (ab73637)

Overview

  • Product nameHuman Cytokeratin 14 full length protein
  • DescriptionHuman Cytokeratin 14 full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab73637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity> 95 % by SDS-PAGE.
    ab73637 is purified by proprietary chromatographic techniques. Purity is greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Additional NotesReconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCI, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 9.5M Urea, 30mM Tris HCl, 2mM EDTA, 10mM methylammonium chloride, pH 8

  • ReconstitutionReconstitute in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

General info

  • Alternative names
    • CK 14
    • CK-14
    • ck14
    • Cytokeratin 14
    • Cytokeratin-14
    • Cytokeratin14
    • Dowling Meara
    • ebs3
    • ebs4
    • Epidermolysis bullosa simplex
    • k14
    • K1C14_HUMAN
    • Keratin
    • Keratin 14
    • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
    • Keratin type I cytoskeletal 14
    • Keratin-14
    • Keratin14
    • Koebner
    • Krt 14
    • krt14
    • NFJ
    • OTTHUMP00000164624
    • type I cytoskeletal 14
    see all
  • FunctionThe nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • Tissue specificityDetected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • Involvement in diseaseDefects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Cellular localizationCytoplasm. Nucleus. Expressed in both as a filamentous pattern.
  • Information by UniProt

Human Cytokeratin 14 full length protein images

  • All lanes : Anti-Cytokeratin 14 [LL002] antibody (ab7800) at 2 µg/ml

    Lane 1 : Human Cytokeratin 14 full length protein (ab73637) at 0.1 µg
    Lane 2 : Human Cytokeratin 14 full length protein (ab73637) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Human Cytokeratin 14 full length protein (ab73637)

ab73637 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and interest in our products. I can confirm that ab73637:Human Cytokeratin 14 full length protein is not tagged and the filament reconstitution is a recommended application thus our Abpromise applies.
I hope this helps an...

Read More

Thank you for your reply.

For Cytokeratin 14, I would recommend the rabbit polyclonal antibody ab48937. This antibody is validated for use in ELISA on human samples, and is predicted to react with mouse. Since this antibody has not been valid...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"