Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||1 x 50ml|
|Cytomegalovirus (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Cytomegalovirus anti-IgG HRP Conjugate||1 x 12ml|
|Cytomegalovirus IgG Negative Control||Blue cap||1 x 100µl|
|Cytomegalovirus IgG Positive Control||Purple cap||1 x 100µl|
|Cytomegalovirus IgG Standard 0 - 0 U/mL||Natural cap||1 x 100µl|
|Cytomegalovirus IgG Standard 1 - 1.2 U/mL||Yellow cap||1 x 100µl|
|Cytomegalovirus IgG Standard 2 - 6 U/mL||Red cap||1 x 100µl|
|Cytomegalovirus IgG Standard 3 - 18 U/mL||Green cap||1 x 100µl|
|IgG Sample Diluent||1 x 22ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108639 in the following tested applications.
|ELISA||Use at an assay dependent dilution.|