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Abcam’s anti-Cytomegalovirus (CMV) IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the qualitative measurement of IgG class antibodies against Cytomegalovirus in Human serum and plasma.
A 96-well plate has been precoated with Cytomegalovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Cytomegalovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Cytomegalovirus IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Cytomegalovirus (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Cytomegalovirus anti-IgG HRP Conjugate||Black cap||1 x 20ml|
|Cytomegalovirus IgG Cut-off Control||Green cap||1 x 3ml|
|Cytomegalovirus IgG Negative Control||Blue cap||1 x 2ml|
|Cytomegalovirus IgG Positive Control||Red cap||1 x 2ml|
|IgG Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108724 has not yet been referenced specifically in any publications.