Overview

Description

  • NatureRecombinant
  • SourceWheat germ
  • Amino Acid Sequence
    • AccessionQ92904
    • SpeciesHuman
    • Molecular weight58 kDa including tags
    • Amino acids1 to 296

Specifications

Our Abpromise guarantee covers the use of ab112279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityuseful for Antibody Production and Protein Array
  • Applications

    Western blot

    SDS-PAGE

    ELISA

    Peptide Array

  • FormLiquid
  • Additional NotesBest use within three months from the date of receipt of this protein.useful for Antibody Production and Protein Array
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione
    Note: Reduced glutathione

General info

  • Alternative names
    • DAZ homolog
    • DAZ like autosomal
    • DAZ-like autosomal
    • DAZH
    • DAZL
    • DAZL 1
    • DAZL_HUMAN
    • DAZL1
    • DAZLA
    • Deleted in azoospermia like
    • Deleted in azoospermia like 1
    • Deleted in azoospermia like autosomal
    • Deleted in azoospermia-like
    • Deleted in azoospermia-like 1
    • Germline specific RNA binding protein
    • MGC26406
    • Spermatogenesis gene on the Y like autosomal
    • SPGY like autosomal
    • SPGY-like-autosomal
    • SPGYLA
    • Tpx2
    see all
  • FunctionRNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • DomainThe DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
  • Target information above from: UniProt accession Q92904 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human DAZL full length protein images

  • 12.5% SDS-PAGE Stained with Coomassie Blue with recombinant protein.
  • Anti-DAZL antibody (ab34139) at 1 µg/ml + Human DAZL full length protein (ab112279) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Human DAZL full length protein (ab112279)

ab112279 has not yet been referenced specifically in any publications.

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