Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • AccessionP27707
    • SpeciesHuman
    • SequenceMRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMATPPKRSCPSFSA SSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQST QDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNG KLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNN QFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHES WLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL
    • Molecular weight35 kDa including tags
    • Amino acids1 to 260
    • TagsHis tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab99520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry
    MALDI-TOF
  • Purity> 90 % by SDS-PAGE.
    ab99520 was purified using conventional chromatography.
  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM EDTA, 1mM DTT, 0.1mM PMSF, pH 7.5

General info

  • Alternative names
    • DCK
    • DCK protein
    • DCK_HUMAN
    • Deoxycytidine kinase
    • Deoxynucleoside kinase
    • EC 2.7.1
    • EC 2.7.1.74
    • MGC117410
    • MGC138632
    • OTTHUMP00000219118
    • OTTHUMP00000219119
    see all
  • FunctionRequired for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.
  • Sequence similaritiesBelongs to the DCK/DGK family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession P27707 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Human DCK full length protein images

  • 15% SDS-PAGE analysis of 3µg ab99520.

References for Human DCK full length protein (ab99520)

ab99520 has not yet been referenced specifically in any publications.

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