Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceTLSVVGPHPKPCIL
    • Amino acids97 to 110

Specifications

Our Abpromise guarantee covers the use of ab23038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names
    • Ddx58
    • DDX58_HUMAN
    • DEAD (Asp Glu Ala Asp) box polypeptide 58
    • DEAD (Asp Glu Ala Asp/His) box polypeptide
    • DEAD box protein 58
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1
    • DKFZp434J1111
    • DKFZp686N19181
    • FLJ13599
    • Probable ATP dependent RNA helicase DDX58
    • Probable ATP-dependent RNA helicase DDX58
    • Retinoic acid inducible gene 1 protein
    • Retinoic acid-inducible gene 1 protein
    • Retinoic acid-inducible gene I protein
    • RIG I
    • Rig-1
    • RIG-I
    • RIG1
    • rigi
    • RLR 1
    • RNA helicase
    • RNA helicase RIG I
    • SGMRT2
    see all
  • FunctionInvolved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
  • Tissue specificityPresent in vascular smooth cells (at protein level).
  • Sequence similaritiesBelongs to the helicase family.
    Contains 2 CARD domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • DomainThe repressor domain controls homomultimerization and interaction with MAVS.
    The helicase domain is responsible for dsRNA recognition.
    The 2 CARD domains are responsible for interaction with and signaling through MAVS.
    The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
  • Post-translational
    modifications
    Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
    Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
  • Cellular localizationCytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
  • Information by UniProt

References for Human DDX58 peptide (ab23038)

ab23038 has not yet been referenced specifically in any publications.

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