Abcam’s anti-Dengue virus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Dengue virus in Human serum and plasma.
A 96-well plate has been precoated with Dengue virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Dengue virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Dengue virus IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Dengue virus (IgM) Coated Microplate (12 x 8 wells)||1 unit|
|Dengue virus anti-IgM HRP Conjugate||colored red; black cap||1 x 20ml|
|Dengue virus IgM Cut-off Control||colored yellow; green cap||1 x 3ml|
|Dengue virus IgM Negative Control||colored yellow; blue cap||1 x 2ml|
|Dengue virus IgM Positive Control||colored yellow; red cap||1 x 2ml|
|IgM Sample Diluent||colored green; white cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108729 has not yet been referenced specifically in any publications.