Overview

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab24556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • DCR
    • DCR1
    • Dicer
    • Dicer 1 ribonuclease type III
    • DICER_HUMAN
    • DICER1
    • Double-strand-specific ribonuclease
    • Endoribonuclease Dicer
    • Helicase MOI
    • Helicase with RNase motif
    • HERNA
    • KIAA0928
    • MNG1
    • RMSE2
    see all
  • FunctionDouble-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Involvement in diseasePleuropulmonary blastoma
    Goiter multinodular 1, with or without Sertoli-Leydig cell tumors
    Rhabdomyosarcoma, embryonal, 2
    DICER1 mutations have been found in uterine cervix embryonal rhabdomyosarcoma, primitive neuroectodermal tumor, Wilms tumor, pulmonary sequestration and juvenile intestinal polyp (PubMed:21882293). Somatic missense mutations affecting the RNase IIIb domain of DICER1 are common in non-epithelial ovarian tumors. These mutations do not abolish DICER1 function but alter it in specific cell types, a novel mechanism through which perturbation of microRNA processing may be oncogenic (PubMed:22187960).
  • Sequence similaritiesBelongs to the helicase family. Dicer subfamily.
    Contains 1 Dicer dsRNA-binding fold domain.
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 PAZ domain.
    Contains 2 RNase III domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt

References for Human Dicer peptide (ab24556)

ab24556 has not yet been referenced specifically in any publications.

Product Wall

The peptide immunogen was xxxxxxxx which corresponds to xxxxxxxx of the immature unprocessed sequence.

I would therefore suggest you use the following blocking peptide: Dicer peptide (ab24556)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"