Overview

  • Product name
    Human DKK3 ELISA Kit (Dickkopf-3)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 3 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    > 85 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.23 85% - 105%
    Serum 86.69 77% - 96%
    Plasma 89.24 80% - 98%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DKK3 (Dickkopf-3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK3 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human DKK3 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human DKK3 2 vials
    DKK3 Microplate (12 x 8 well strips) 1 unit
    Recombinant Human DKK3 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    DKK3, like DKK1, DKK2, and DKK4, possesses an N terminal signal peptide and 2 conserved cysteine rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin type proteases.
  • Cellular localization
    Secreted
  • Alternative names
    • AW061014
    • C87148
    • Dickkopf (Xenopus laevis) homolog 3
    • Dickkopf 3
    • Dickkopf homolog 3 (Xenopus laevis)
    • Dickkopf related protein 3
    • HDkk 3
    • Regulated in glioma
    • REIC
    • RIG
    • RIG like 5 6
    • RIG like 7 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100502.

  • Representative Standard Curve using ab100502.

Protocols

References

This product has been referenced in:
  • Shin JM  et al. The expression pattern and functional role of REIC/Dkk-3 in the development of cutaneous squamous cell carcinoma. J Dermatol Sci 84:88-96 (2016). Read more (PubMed: 27354306) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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