Human DKK4 ELISA Kit (Dickkopf-4) (ab100503)

Overview

  • Product nameHuman DKK4 ELISA Kit (Dickkopf-4)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 109.6 97% - 121%
    Serum 95.59 85% - 105%
    Plasma 82.62 69% - 91%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DKK4 (Dickkopf-4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human DKK4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human DKK4 coated on a 96-well plate. Standards and samples are pipetted into the wells and DKK4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human DKK4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DKK4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceDKK4, like DKK1, DKK2, and DKK3, possesses an N terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis detected no expression of DKK4, but RT-PCR analysis detected DKK4 expression in cerebellum, T cell, esophagus, and lung cDNA libraries. DKK4 blocks Xenopus Wnt8, Wnt3a, and Wnt2b, but not Dsh or Fz8, induction of a secondary axis in frog embryos, indicating that DKKs antagonize WNT function upstream of WNT receptors.
  • Alternative names
    • Dickkopf 4
    • Dickkopf gene 4
    • Dickkopf homolog 4
    • Dickkopf related protein 4
    • Dickkopf4
    • DKK 4
    • hDkk4
    • MGC129562
    • MGC129563
    • MGC25705
    • RGD1563172
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human DKK4 ELISA Kit (Dickkopf-4) images

  • Representative Standard Curve using ab100503.

Protocols

References for Human DKK4 ELISA Kit (Dickkopf-4) (ab100503)

ab100503 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Our testing data indicates that kit ab100503 shows less than 2% cross-reactivity with mouse. I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"