• Product name
    Human DMP1 ELISA Kit
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Serum, Plasma, Cell culture media
  • Assay type
  • Sensitivity
    80 pg/ml
  • Range
    80 pg/ml - 40000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 77.96 69% - 87%
    Plasma 82.32 73% - 93%
    Cell culture media 75.63 67% - 83%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's DMP1 Human ELISA Kit (ab193699) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DMP1 in sera, plasma and cell culture supernatants.

    This assay employs an antibody specific for human DMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures DMP1 present in the samples. The wells are washed and biotinylated anti-human DMP1 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of DMP1 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated Human DMP-1 detection antibody (lyophilized) 2 vials
    Human DMP-1 standards 2 vials
    Pre-coated Human DMP-1 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
  • Tissue specificity
    Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.
  • Involvement in disease
    Hypophosphatemic rickets, autosomal recessive, 1
  • Post-translational
    Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif.
  • Cellular localization
    Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
  • Information by UniProt
  • Alternative names
    • Dentin matrix acidic phosphoprotein 1
    • Dentin matrix protein 1
    • DMP-1
    • Dmp1
    • DMP1_HUMAN
    see all
  • Database links


Our Abpromise guarantee covers the use of ab193699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.




ab193699 has not yet been referenced specifically in any publications.

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