Overview

  • Product nameHuman DR5 ELISA Kit (CD262)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 483pg/ml 7 1.39%
    Sample B 8 117pg/ml 4 3.23%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 9 679pg/ml 45 6.58%
    Sample B 9 186pg/ml 14 7.44%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    31.25 pg/ml - 1000 pg/ml
  • Assay time
    2h 30m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s DR5 (CD262) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of DR5 in Human serum, plasma, buffered solutions and cell culture media.

    A monoclonal antibody specific for DR5 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known DR5 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for DR5 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of DR5 present in the samples. 

    This kit will recognize both endogenous and recombinant Human DR5.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceDR5 is a member of the TNF-receptor superfamily, and contains an intracelluar death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
  • Alternative names
    • Death receptor 5
    • KILLER
    • TNFRSF10B
    • TRAIL R2
    • TRAIL receptor 2
    • TRICK2
    • TRICKB
    • ZTNFR9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human DR5 ELISA Kit (CD262) images

  • Representative Standard Curve using ab46013

Protocols

References for Human DR5 ELISA Kit (CD262) (ab46013)

ab46013 has not yet been referenced specifically in any publications.

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