Overview

  • Product nameHuman Drosha peptide

Description

  • NatureSynthetic
  • SourceCHO cells

Specifications

Our Abpromise guarantee covers the use of ab12307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Drosha antibody - ChIP Grade (ab12286)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Information available upon request.

General Info

  • Alternative names
    • DROSHA
    • Drosha double stranded RNA specific endoribonuclease
    • Drosha ribonuclease type III
    • Etohi2
    • HSA242976
    • Nuclear RNase III Drosha
    • p241
    • Protein Drosha
    • Putative protein p241 which interacts with transcription factor Sp1
    • Putative ribonuclease III
    • RANSE3L
    • Ribonuclease 3
    • Ribonuclease III
    • Ribonuclease III nuclear
    • Ribonuclease type III nuclear
    • RibonucleaseIII
    • RN 3
    • RN3
    • RNase 3
    • RNase III
    • RNase3
    • RNASE3L
    • RNaseIII
    • RNASEN
    • RNC_HUMAN
    see all
  • FunctionRibonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.
  • DomainThe 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
  • Cellular localizationNucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt

References for Human Drosha peptide (ab12307)

This product has been referenced in:
  • Nakamura T  et al. Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing. Proc Natl Acad Sci U S A 104:10980-5 (2007). Read more (PubMed: 17581865) »

See 1 Publication for this product

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