Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab41752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-EAAT2 antibody (ab41621)

  • Purity
    > 90 % n/a.

  • Form
    Lyophilised
  • Additional notes

    Reconstitution instructions:

     -  First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.

    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.

    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.

    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.

    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • EAA2_HUMAN
    • EAAT2
    • Excitatory amino acid transporter 2
    • Excitotoxic amino acid transporter 2
    • Glial high affinity glutamate transporter
    • GLT 1
    • GLT1
    • Glutamate aspartate transporter II
    • Glutamate transporter 1
    • Glutamate/aspartate transporter II
    • Slc1a2
    • Sodium dependent glutamate aspartate transporter 2
    • Sodium-dependent glutamate/aspartate transporter 2
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2
    • Solute carrier family 1 glial high affinity glutamate transporter member 2
    • Solute carrier family 1 member 2
    see all
  • Function
    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities
    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Potapenko ES  et al. Altered astrocyte glutamate transporter regulation of hypothalamic neurosecretory neurons in heart failure rats. Am J Physiol Regul Integr Comp Physiol 303:R291-300 (2012). Read more (PubMed: 22696576) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Other
Predicted band size would be 62 kDa.
however observed band size for cow liver is less then 62 kDa.
Loading amount of each lane : 60ug of cow liver
1 lane: Fresh Primary antibody (ab41621)
2 lane: Fresh Primary antibody with 45 min incubation at RT
3 lane: only Secondary antibody (without primary antibody)
4 lane: Primary antibody : Peptide = 1 : 0.01with 45 min incubation at RT
5 lane: Primary antibody : Peptide = 1 : 0.001with 45 min incubation at RT
6 lane: Primary antibody : Peptide = 1 : 0.0001with 45 min incubation at RT
Username

Mr. Kwangwon Son

Verified customer

Submitted Nov 16 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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