Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884)

Overview

  • Product nameHuman Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4)
  • Product overview

    ab109884 is a Human Embyonic Stem Cell Marker Panel containing 50µg of Oct4 rabbit polyclonal, 50µg of Tra-1-60 mouse monoclonal, 50µl of Nanog rabbit polyclonal antibody, 50µg of SOX2 rabbit polyclonal antibody and 50µl of SSEA4 mouse monoclonal antibody antibodies.

    The Human Embryonic Stem Cell Marker Panel is designed for the validation and characterization of cultured or newly derived human embryonic stem (ES) cell lines. The panel contains five antibodies to well known markers of human ES cells (Oct4, Nanog, SOX2, SSEA4 and Tra-1-60). Embryonic stem cells are isolated from the inner cell mass of the early blastocyst. They have two properties that make them unique. Firstly, under defined conditions they can self-renew indefinitely and secondly they are pluripotent, which means they can differentiate into all three germ layers (ectoderm, mesoderm and endoderm).

Properties

    Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) images

    • This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
    • ICC/IF image of Sox2 (ab97959) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.01% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1/500 dilution) overnight at +4°C.
    • NTERA2 Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
    • ICC/IF image of Nanog (ab21624) stained Human Embryonic Stem Cells. The cells were paraformaldehyde fixed and then incubated in 1% serum / 0.1% triton / 0.1% BSA in PBS for 30min to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21624, 1/300 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/500 dilution.

    References for Human Embryonic Stem Cell Marker Panel (Oct4, Nanog, Tra-1-60, SOX2 and SSEA4) (ab109884)

    ab109884 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your recent telephone enquiry.

    I am pleased to provide the ICC/IF protocol used for the ab109884 co-staining images. I hope this will be helpful. Please note, this is a guideline only and may require some further individual opt...

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    Thank you for your message which has been forwarded to the scientific support team.

    I will be pleased to help with your questions. In order to do so, I would appreciate if you could confirm the product number of the stem cell marker panel yo...

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    Thank you for your enquiry and your interest in our products.

    ab109884 is a Human Embyonic Stem Cell Marker Panel containing 5 different primary antibodies - only. The secondary antibodies are not included in this panel:

    50µg ...

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    Thank you very much for your email.

    The antibodies of this stem cells panel are fully characterized and tested in the applications as suggested on the datasheet. I can confirm that there no specific protocol is needed for the antibodies; thes...

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    Thank you for your enquiry and your interest. I regret to inform you that we are unable to change the components of this kit. However, if your customer would like to use different antibodies as markers, the preferred antibodies can be purchased...

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    Thank you for your enquiry. I would be pleased to provide a quotation for you. In order to do so, I would appreciate if you could confirm which country you are contacting us from?  I am sorry I am not able to find this in the correspondence or from...

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    Thank you for contacting us. We only have following Human ESC ab109884 product in catalogue. http://www.abcam.com/Human-Embryonic-Stem-Cell-Marker-Panel-Oct4-Nanog-Tra-1-60-SOX2-and-SSEA4-ab109884.html We unfortunately do not have...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"