Human ENO3 full length protein (ab113127)
- Product nameHuman ENO3 full length proteinSee all ENO3 proteins and peptides ...
- DescriptionRecombinant, His tag
- SourceE. coli
- Amino Acid Sequence
- SequenceMGSSHHHHHHSSGLVPRGSHMAMQKIFAREILDSRGNPTV EVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGK GVLKAVENINSTLGPALLQKKLSVADQEKVDKFMIELDGT ENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN PDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKE AMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILEN NEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLD FKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDD WATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCL LLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTF IADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK AIFAGRKFRNPKAK
- Molecular weight49 kDa including tags
- Amino acids1 to 434
- TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab113127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Mass spectrometryMALDI-TOF
- Purity> 95
% by SDS-PAGE.
ab113127 was purified using conventional chromatography.
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.32% Tris HCl, 0.02% DTT, 20% Glycerol, 0.58% Sodium chloride
- 2 phospho D glycerate hydrol yase
- 2 phospho D glycerate hydrolyase
- 2-phospho-D-glycerate hydro-lyase
- Beta enolase
- Enolase 3
- Enolase 3 (beta muscle)
- Muscle specific enolase
- Muscle-specific enolase
- Skeletal muscle enolase
- FunctionAppears to have a function in striated muscle development and regeneration.
- Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
- Involvement in diseaseDefects in ENO3 are the cause of glycogen storage disease type 13 (GSD13) [MIM:612932]. A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis.
- Sequence similaritiesBelongs to the enolase family.
- Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
- Cellular localizationCytoplasm. Localized to the Z line. Some colocalization with CKM at M-band.
References for Human ENO3 full length protein (ab113127)
ab113127 has not yet been referenced specifically in any publications.