Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab48606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-ENT1 antibody (ab48607)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Equilibrative NBMPR-sensitive nucleoside transporter
    • equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter
    • Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
    • Equilibrative nucleoside transporter 1
    • es-type
    • MGC1465
    • MGC3778
    • Nucleoside transporter
    • Nucleoside transporter, es-type
    • OTTHUMP00000016506
    • OTTHUMP00000016507
    • OTTHUMP00000016508
    • OTTHUMP00000016509
    • OTTHUMP00000016510
    • OTTHUMP00000016511
    • OTTHUMP00000016512
    • S29A1_HUMAN
    • Slc29a1
    • solute carrier family 29 (equilibrative nucleoside transporter), member 1
    • solute carrier family 29 (nucleoside transporters), member 1
    • Solute carrier family 29 member 1
    see all
  • Function
    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
  • Tissue specificity
    Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
  • Sequence similarities
    Belongs to the SLC29A transporter family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
  • Information by UniProt

Images

  • All lanes : Anti-ENT1 antibody (ab48607) at 2 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Human heart tissue lysate - total protein (ab29431) with Human ENT1 peptide (ab48606) at 2 µg/ml
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human ENT1 peptide (ab48606) at 2 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

References

ab48606 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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