Human Anti-Entamoeba histolytica IgG ELISA Kit (ab108734)

Overview

  • Product nameHuman Anti-Entamoeba histolytica IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 7 2.1%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 6 3.9%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.

     

    A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    Entamoeba histolytica (IgG) Cut-off Control colored yellow; green cap 1 x 3ml
    Entamoeba histolytica (IgG) Negative Control colored yellow; blue cap 1 x 2ml
    Entamoeba histolytica (IgG) Positive Control colored yellow; red cap 1 x 2ml
    Entamoeba histolytica Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit
    Entamoeba histolytica Protein A Conjugate colored blue; black cap 1 x 20ml
    IgG Sample Diluent colored yellow; white cap 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab108734 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Protocols

    References for Human Anti-Entamoeba histolytica IgG ELISA Kit (ab108734)

    ab108734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"