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Abcam’s anti-Entamoeba histolytica IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma.
A 96-well plate has been precoated with Entamoeba histolytica antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a Protein A peroxidase labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Entamoeba histolytica-specific antibodies. TMB is then catalyzed by the Protein A to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Entamoeba histolytica IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Entamoeba histolytica (IgG) Cut-off Control||colored yellow; green cap||1 x 3ml|
|Entamoeba histolytica (IgG) Negative Control||colored yellow; blue cap||1 x 2ml|
|Entamoeba histolytica (IgG) Positive Control||colored yellow; red cap||1 x 2ml|
|Entamoeba histolytica Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Entamoeba histolytica Protein A Conjugate||colored blue; black cap||1 x 20ml|
|IgG Sample Diluent||colored yellow; white cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108734 has not yet been referenced specifically in any publications.