The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-EPLIN antibody (ab50196)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Epithelial protein lost in neoplasm
Epithelial protein lost in neoplasm beta
LIM domain and actin binding 1
LIM domain and actin binding protein 1
LIM domain and actin-binding protein 1
Sterol regulatory element binding protein 3
FunctionBinds to actin monomers and filaments. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments.
Tissue specificityHighly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform Alpha abundant in epithelial cells from mammary gland, prostate and in normal oral keratinocytes. Low levels in aortic endothelial cells and dermal fibroblasts. Not detectable in myocardium.
DomainContains at least 2 actin-binding domains, one on each side of the LIM domain. Both domains bind actin monomers and filaments. The C-terminal domain binds filaments more efficiently than the N-terminus.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Cytoplasm > cytoskeleton. This cytoskeletal protein co-localizes with actin stress fibers and focal adhesion plaques.