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Abcam’s anti-Epstein Barr virus (EBV-VCA) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Epstein Barr virus in Human serum and plasma.
A 96-well plate has been precoated with Epstein Barr virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Epstein Barr virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Epstein Barr virus IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|Epstein Barr virus (IgM) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Epstein Barr virus anti-IgM HRP Conjugate||colored red; black cap||1 x 20ml|
|Epstein-Barr Virus IgM Negative Control||colored yellow; blue cap||1 x 2ml|
|Epstein-Barr Virus IgM Positive Control||colored yellow; red cap||1 x 2ml|
|Epstein-Barr-Virus IgM Cut-off Control||colored yellow; green cap||1 x 3ml|
|IgM Sample Diluent||White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108732 has not yet been referenced specifically in any publications.