Overview

  • Product name
    Human Erythropoietin ELISA Kit (EPO)
    See all Erythropoietin (EPO) kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8 6.2%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.4 mIU/ml
  • Range
    1.6 mIU/ml - 100 mIU/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 81 71% - 93%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Erythropoietin (EPO) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Erythropoietin concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    Erythropoietin specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotinylated Erythropoietin detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and unbound conjugates are then washed away using was buffer. TMB is then added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Erythropoietin captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Erythropoietin standard curve: mean of duplicates (+/- SD)
  • Human Erythropoietin measured in biological fluids showing quantity (mIU) per mL of tested sample. Samples diluted 1-4 fold.

  • Representative Standard Curve using ab119522.

Protocols

References

This product has been referenced in:
  • Hansen HG  et al. Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells. Sci Rep 5:18016 (2015). WB . Read more (PubMed: 26657798) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your enquiry regarding ab119522.

This product has been designed to detect human Erythropoietin (EPO) and it has only been tested on human samples; we do not have any information regarding its cross-reactivity with other species....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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