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|Sample type||Average %||Range|
|Serum||101.3||18pg/ml - 810pg/ml|
Abcam’s Estradiol E2 in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Estradiol E2 in Human serum.
A 96-well plate has been pre-coated with goat anti-rabbit IgG. Samples, standards and the rabbit anti-Estradiol E2 Reagent and Estradiol E2-HRP conjugate are added to the wells, where Estradiol E2 in the sample competes with the added Estradiol E2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Estradiol E2 in the sample and the intensity is measured at 450 nm.
|Components||Identifier||1 x 96 tests|
|Estradiol E2 Control 1 - 13.9pg/ml||1 x 0.5ml|
|Estradiol E2 Control 2 - 269 pg/ml||1 x 0.5ml|
|Estradiol E2 Standard 1 - 0 pg/mL||1 x 0.5ml|
|Estradiol E2 Standard 2 - 10 pg/mL||1 x 0.5ml|
|Estradiol E2 Standard 3 - 30 pg/mL||1 x 0.5ml|
|Estradiol E2 Standard 4 - 100 pg/mL||1 x 0.5ml|
|Estradiol E2 Standard 5 - 300 pg/mL||1 x 0.5ml|
|Estradiol E2 Standard 6 - 1000 pg/mL||1 x 0.5ml|
|Estradiol E2-HRP Conjugate Reagent||Blue colour||1 x 12ml|
|Goat anti-rabbit IgG Coated Microplate (12 x 8 wells)||1 x 96 tests|
|Rabbit Anti-Estradiol E2 Reagent||Pink colour||1 x 7ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
ab108640 has not yet been referenced specifically in any publications.