The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
3T3-L1 lipid-binding protein
Adipocyte lipid binding protein
Adipocyte lipid-binding protein
Adipocyte protein AP2
Adipocyte-type fatty acid-binding protein
Epididymis secretory protein Li 104
Fatty acid binding protein 4
Fatty acid binding protein 4 adipocyte
Fatty acid binding protein adipocyte
Fatty acid-binding protein
Fatty acid-binding protein 4
HEL S 104
Myelin P2 protein homolog
P2 adipocyte protein
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.