Human Factor D ELISA Kit (ab99969)
Key features and details
- Sensitivity: 4 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor D ELISA Kit
See all Factor D/CFD kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 4 pg/ml -
Recovery
< 130 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 126.4 115% - 133% Serum 130.3 118% - 139% Plasma 131.1 107% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-human Adipsin 2 vials Factor D Microplate (12 strips x 8 wells) 1 unit Recombinant human Fatcor D Standards (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Adipsin
- Adipsin/complement factor D
- Adn
see all -
Database links
- Entrez Gene: 1675 Human
- Omim: 134350 Human
- SwissProt: P00746 Human
- Unigene: 155597 Human
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab99969 has been referenced in 3 publications.
- Dollerup OL et al. Effects of Nicotinamide Riboside on Endocrine Pancreatic Function and Incretin Hormones in Nondiabetic Men With Obesity. J Clin Endocrinol Metab 104:5703-5714 (2019). PubMed: 31390002
- Poveda NE et al. Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia. Sci Rep 6:20073 (2016). PubMed: 26832661
- Sweigard JH et al. Inhibition of the alternative complement pathway preserves photoreceptors after retinal injury. Sci Transl Med 7:297ra116 (2015). PubMed: 26203084