Overview

  • Product nameHuman Factor D ELISA Kit
    See all Factor D kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Recovery

    < 130 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 126.4 115% - 133%
    Serum 130.3 118% - 139%
    Plasma 131.1 107% - 140%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Factor D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Factor D in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Factor D coated on a 96-well plate. Standards and samples are pipetted into the wells and Factor D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Factor D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Factor D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionFactor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Involvement in diseaseComplement factor D deficiency (CFDD) [MIM:613912]: An immunologic disorder characterized by increased susceptibility to bacterial infections, particularly Neisseria infections, due to a defect in the alternative complement pathway. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Adipsin
    • ADN
    • C3 convertase activator
    • CFAD_HUMAN
    • CFD
    • Complement factor D
    • Complement factor D preproprotein
    • D component of complement
    • DF
    • PFD
    • Properdin factor D
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab99969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Factor D ELISA Kit images

  • Representative Standard Curve using ab99969.

  • Representative Standard Curve using ab99969.

Protocols

References for Human Factor D ELISA Kit (ab99969)

ab99969 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.
Protease inhibitors are not necessary for testing serum or plasma samples. We only recommend using inhibitors when testing lysate samples.
Adding protease inhibitors into serum / plasma will not interfere with the...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"