Abcam’s Factor H Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Factor H in urine, saliva, milk, plasma, serum, cerebrospinal fluid and cell culture supernatants.
A Factor H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor H captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|70X Biotinylated Human Factor H Antibody||1 x 90µl|
|Chromogen Substrate||1 x 8ml|
|Factor H Microplate (12 x 8 well strips)||1 unit|
|Factor H Standard||2 vials|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab137975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve with background signal subtracted (duplicates; +/- SD).
Factor H measured in culture supernatants (tested at dilution range 1/1-1/30; duplicates +/- SD).
Factor H measured in biological fluids (duplicates +/- SD). Human serum and plasma were tested at dilution range of 1/50000-1/1500000. Other bilogical fluids were tested at 1/1-1/500 and gave measurable values (milk: 400, saliva: 200, CSF: 2000 and urine: 40 ng per mL).
Representative Standard Curve using ab137975