Overview

  • Product name
    Human Factor IX ELISA Kit
    See all Factor IX kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Human serum 9 4%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 6%
  • Sample type
    Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.23 ng/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 108 86% - 130%
    Heparin Plasma 103 85% - 119%
    EDTA Plasma 98 78% - 107%
    Citrate Plasma 104 94% - 121%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Abcam’s Factor IX in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein in human serum and plasma samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Factor IX, also known as Christmas Factor, is a 52 kDa zymogen of plasma serine proteases that initiates coagulation through activation by Tissue Factor (TF) and Factor VIIa. The activated form of Factor IX, known as Factor IXa, further activates an intrinsic activation complex that consists of factor X and factor VIIIa in the presence of Ca (2+) ions, and phospholipids. Mutations that cause a deficiency in Factor IX lead to x-linked hemophilia B, whereas overproduction of Factor IX has been shown to be a risk factor in venous thromboembolism (VTE). In 2013 the Food and Drug Administration approved the use of recombinant factor IX as a prophylactic treatment of patients with hemophilia B.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer PT (ab206977) 1 x 20ml
    ab221824 - Antibody Diluent 4BI 1 x 6ml
    Factor IX Capture Antibody (Lyophilized) 2 vials
    Factor IX Detector Antibody (Lyophilized) 2 vials
    Factor IX Human Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Substrate 1 x 12ml
  • Research areas
  • Relevance
    Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.
  • Cellular localization
    Secreted
  • Alternative names
    • Cf-9
    • Cf9
    • Christmas factor
    • Coagulation factor IX
    • F9 p22
    • Factor 9
    • Factor IX F9
    • FIX
    • FIX F9
    • HEMB
    • P19
    • Plasma thromboplastic component
    • Plasma Thromboplastin Component
    • PTC
    • THPH8
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab188393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (400 fold). The mean level of Factor IX is 1,240 ng/mL with a range of 246 – 3,994 ng/mL and a standard deviation of 1,193 ng/mL.

Protocols

References

This product has been referenced in:
  • Huang L  et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol 19:4 (2018). Read more (PubMed: 29334995) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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