Overview

  • Product name
    Human Factor VII ELISA Kit
    See all Factor VII kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.6%
  • Sample type
    Cell culture supernatant, Saliva, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 6 ng/ml
  • Range
    6.25 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Factor VII Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor VII concentrations in Human cell culture supernatants, serum and plasma.


    A Factor VII specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor VII specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor VII captured in plate. This assay recognizes FVII and FVIIa.


    Get higher sensitivity in only 90 minutes with Human Factor VII ELISA Kit (ab190810) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Factor VII Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Factor VII Microplate (12 x 8 well strips) 1 unit
    Factor VII Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Factor VII deficiency
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Cf7
    • Coagulation factor VII
    • Coagulation factor VII (serum prothrombin conversion accelerator)
    • Eptacog alfa
    • F7
    • F7 deficiency
    • FA7_HUMAN
    • Factor VII deficiency
    • Factor VII heavy chain
    • FVII
    • FVII coagulation protein
    • Hypoproconvertinemia
    • Proconvertin
    • Serum prothrombin conversion accelerator
    • SPCA
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108829.

Protocols

References

ab108829 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1. The standard provided in the kit is purified factor VII. No activation is necessary for this standard.
2. The antibodies used in this kit have not been mapped. The epitopes are unknown.

Thank you for your enquiry.

I can confirm this kit has not been specifically tested on supernatant from hepatocytes so the dilution will need to be individually optimized. Each cell culture sample is different. so it is not possible to sugges...

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Thank you for your enquiry.

Unfortunately, the kit has no cross-reactivity against mouse. Please refer page 15 of the protocol booklet on the datasheet of ab108829.

I hope this information will still be helpful. If there is anythi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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