Overview

  • Product name
    Human Factor XI ELISA Kit
    See all Factor XI kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.56 ng/ml - 50 ng/ml
  • Recovery

    97.5 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI levels in plasma, serum, and cell culture supernatants.

    A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    70X Biotinylated Human Factor XI Antibody 1 x 90µl
    Chromogen Substrate 1 x 8ml
    Factor XI Microplate (12 x 8 well strips) 1 unit
    Factor XI Standard 2 vials
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
  • Tissue specificity
    Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
  • Involvement in disease
    Factor XI deficiency (FA11D) [MIM:612416]: A hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
    Contains 4 apple domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Coagulation factor XI
    • Coagulation factor XIa light chain
    • F11
    • FA11_HUMAN
    • FXI
    • Plasma thromboplastin antecedent
    • PTA
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108834.

Protocols

References

ab108834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1. At the power form, it should be good for around 1 year.
2. After dissolve in buffer, the standard need to be aliquot and store at -80 degree if possible. If store at -80, the standard can be good for at le...

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Thank you for contacting us.

The plate can last for at least one year from the date received the kit as long as the pouch un-open. If the pouch has been opened, please keep it in the desicator if you have one. Keep dry is very important. If y...

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Comme indiqué dans le protocol du kit ELISA facteur XI ab108834 (http://www.abcam.com/ps/products/108/ab108834/documents/ab108834%20Factor%20XI%20Human%20ELISA_v3%20%28Website%29.pdf), il est conseillé d'effectuer 2 ou 3 séries de 7 points de standards...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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